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J. to properly reestablish cell polarity after junction disassembly. Recovery of cellCcell adhesion, transepithelial resistance, and the localization of TJ and AJ markers were all delayed. In contrast, depletion of -catenin caused long-term disruption of junctions. These results indicate that E-cadherin and Cadherin-6 function as a scaffold for the construction of IPI-145 (Duvelisib, INK1197) polarized structures, and they become largely dispensable in mature junctions, whereas -catenin is essential for the maintenance of functional junctions. INTRODUCTION The cadherins are a large family of transmembrane glycoproteins that form homophilic, calcium-dependent interactions with neighboring cells (Takeichi, 1988 ; Gumbiner, 2000 ; Nollet have demonstrated that it is essential from early IPI-145 (Duvelisib, INK1197) embryogenesis through the later stages of organogenesis (Larue (http://www.molbiolcell.org). This article was published online ahead of print in (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E06-05-0471) on November 8, 2006. REFERENCES Adams C. L., Nelson W. J. Cytomechanics of cadherin-mediated cell-cell adhesion. Curr. Opin. Cell Biol. 1998;10:572C577. [PubMed] [Google Scholar]Ando-Akatsuka Y., Yonemura S., Itoh M., Furuse M., Tsukita S. Differential behavior of E-cadherin and occludin in their colocalization with ZO-1 during the establishment of epithelial cell polarity. J. Cell Physiol. 1999;179:115C125. [PubMed] [Google Scholar]Angres B., Barth A., Nelson W. J. Mechanism for transition from initial to stable cell-cell adhesion: kinetic analysis of E-cadherin-mediated adhesion using a quantitative adhesion assay. J. IPI-145 (Duvelisib, INK1197) Cell Biol. 1996;134:549C557. [PMC free article] [PubMed] [Google Scholar]Bershadsky A. Magic touch: how does cell-cell adhesion trigger actin assembly? Trends Cell Biol. 2004;14:589C593. [PubMed] [Google Scholar]Boggon T. J., Murray J., Chappuis-Flament S., Wong E., Gumbiner B. M., Shapiro L. C-cadherin ectodomain structure and implications for cell adhesion mechanisms. Science. 2002;296:1308C1313. [PubMed] [Google Scholar]Braga V. M. Cell-cell adhesion and signalling. Curr. Opin. Cell Biol. 2002;14:546C556. [PubMed] [Google Scholar]Braga V. M., Machesky L. M., Hall A., Hotchin N. A. The small GTPases Rho and Rac are Rabbit polyclonal to Cytokeratin 1 required for the establishment of cadherin-dependent cell-cell contacts. J. Cell Biol. 1997;137:1421C1431. [PMC free article] [PubMed] [Google Scholar]Brummelkamp IPI-145 (Duvelisib, INK1197) T. R., Bernards R., Agami R. A system for stable expression of short interfering RNAs in mammalian cells. Science. 2002;296:550C553. [PubMed] [Google Scholar]Bryant D. M., Stow J. L. The ins and outs of E-cadherin trafficking. Trends Cell Biol. 2004;14:427C434. [PubMed] [Google Scholar]Chen X., Macara I. G. Par-3 controls tight junction assembly through the Rac exchange factor Tiam1. Nat. Cell Biol. 2005;7:262C269. [PubMed] [Google Scholar]Cowin P., Rowlands T. M., Hatsell S. J. Cadherins and catenins in breast cancer. Curr. Opin. Cell Biol. 2005;17:499C508. [PubMed] [Google Scholar]D’Souza-Schorey C. Disassembling adherens junctions: breaking up is hard to do. Trends Cell Biol. 2005;15:19C26. [PubMed] [Google Scholar]Davis M. A., Ireton R. C., Reynolds A. B. A core function for p120-catenin in cadherin turnover. J. Cell Biol. 2003;163:525C534. [PMC free article] [PubMed] [Google Scholar]Drees F., Pokutta S., Yamada S., Nelson W. J., Weis W. I. Alpha-catenin is a molecular switch that binds E-cadherin-beta-catenin and regulates actin-filament assembly. Cell. 2005;123:903C915. [PMC free article] [PubMed] [Google Scholar]Gao L., Joberty G., Macara I. G. Assembly of epithelial tight junctions is negatively regulated by Par6. Curr. Biol. 2002;12:221C225. [PubMed] [Google Scholar]Gavard J., Mege R. M. Once upon a time there was beta-catenin in cadherin-mediated signalling. Biol. Cell. 2005;97:921C926. [PubMed] [Google Scholar]Geiger B., Volberg T., Ginsberg D., Bitzur S., Sabanay I., Hynes R. O. Broad spectrum pan-cadherin antibodies, reactive with the C-terminal 24 amino acid residues of N-cadherin. J. Cell Sci. 1990;97:607C614. [PubMed] [Google Scholar]Gonzalez-Mariscal L., Betanzos A., Nava P., Jaramillo B. E. Tight junction proteins. Prog. Biophys. Mol. Biol. 2003;81:1C44. [PubMed] [Google Scholar]Gonzalez-Mariscal L., Contreras R. G., Bolivar J. J., Ponce A., Chavez De Ramirez B., Cereijido M. Role of calcium in tight junction formation between epithelial cells. Am. J. Physiol. 1990;259:C978CC986. [PubMed] [Google Scholar]Gumbiner B., Stevenson B., Grimaldi A. The role of the cell adhesion molecule uvomorulin in the formation and maintenance of the epithelial junctional complex. J. Cell Biol. 1988;107:1575C1587. [PMC free article] [PubMed] [Google Scholar]Gumbiner B. M. Cell adhesion: the molecular basis of tissue architecture and morphogenesis. Cell. 1996;84:345C357. [PubMed] [Google Scholar]Gumbiner B. M. Regulation of cadherin adhesive activity. J. Cell Biol. 2000;148:399C404. [PMC free article] [PubMed] [Google Scholar]Huber A. H., Weis W. I. The structure of the -catenin/E-cadherin complex and the molecular basis of diverse ligand recognition by beta-catenin. Cell. 2001;105:391C402. [PubMed] [Google Scholar]Ivanov A. I., McCall I. C., Babbin B., Samarin S. N., Nusrat A., Parkos C. A. Microtubules regulate disassembly of epithelial apical junctions. BMC Cell Biol. 2006;7:12. [PMC free article] [PubMed] [Google Scholar]Jaffe S. H., Friedlander D. R., Matsuzaki F., Crossin K. L., Cunningham.