Background Tomato-based food products have health-promoting and disease-preventing effects. resting energy expenditures (REEs); and serum levels of triglyceride (TG), cholesterol, glucose, and hemoglobin A1c were measured. Results Ninety-three women (98%) completed the study. The following parameters showed significant changes, compared with baseline, at study weeks 4 and 8 (mean??standard deviation at baseline, week 4, and week 8): (1) the MSS score improved (9.9 5.2, 8.5 5.0, 8.3 5.0; P?CCL2 TG (150?mg/dL or higher) at baseline (237.8??88.9?mg/dL, 166.7??86.1?mg/dL, 170.9??109.7?mg/dL; P?=?0.0002, Friedman test). Conclusions Tomato juice intake alleviated menopausal symptoms, including stress, increased REEs and heart rate, and lowered high baseline serum TG levels in middle-aged women. Trial registration UMIN-CTRUMIN000011877. Keywords: Menopausal symptoms, Stress, Basal metabolism, Dyslipidemia Background Middle-aged women are not only bothered by the psychological and physical symptoms of menopause, but may also be at elevated risk for cardiovascular illnesses (CVDs), such as for example central weight problems, hypertension, dyslipidemia, and diabetes [1-4], that are partially buy 202591-23-9 induced due to diminished estrogen creation [5]. CVD may be the accurate number 1 reason behind loss of life world-wide, accounting for 17.3 million (30%) fatalities, globally, in 2008 [6]. Many CVDs could possibly be avoided via an reduction of risk elements theoretically, such as cigarette use, unhealthy diet plans, weight problems, high blood circulation pressure, impaired blood sugar tolerance, and elevated lipids [7]; nevertheless, adjustment of types own way of living is difficult often. Tomato juice contains a number of bioactive ingredients, such as for example gamma-aminobutyric acidity (GABA), lycopene, 13-oxo-9,11-octadecadienoic acidity (13-oxo-ODA), and esculeoside A, which might offer physical and emotional health benefits for middle-aged women. For example, GABA reduces psychological stress [8] and lowers blood pressure [9]. Lycopene has been reported to have anti-cancer effects and anti-oxidative effects, and to be effective at mitigating cardiovascular diseases, osteoporosis, and mental disorders [10-14]. Recently, buy 202591-23-9 13-oxo-ODA was shown to lower plasma and hepatic triglyceride (TG) levels in an animal model of obesity [15]. Esculeoside A, a tomato saponin, was also shown to reduce serum levels of TG and cholesterol, and to ameliorate atherosclerotic lesions in ApoE-deficient mice [16]. Compared to new tomatoes, tomato juice has several advantages, including an increased level of extractable lycopene [17], and elevated antioxidant capacity (as a result of activation through the canning (heating) process) [18], and additionally, 13-oxo-ODA is found only in tomato juice [15]. Moreover, a recent research demonstrated that tomato vinegar drink improved blood sugar tolerance in high-fat diet-induced obese mice [19]. In today’s research, we investigated the web aftereffect of tomato juice consumption on a number of wellness variables in middle-aged females, predicated on the hypothesis that anti-oxidative aftereffect of lycopene buy 202591-23-9 and soothing aftereffect of GABA may relieve physical and emotional symptoms of menopause; 13-oxo-ODA and Esculeoside A may boost resting energy expenses (REE) and lower the serum degree of triglyceride. Strategies We executed an open-label, single-arm research on the Menopause Clinic from the Tokyo Oral and Medical University. The analysis process was analyzed and accepted by the Tokyo Medical and Teeth School Review Plank, and written educated consent was from all participants. The study was carried out in accordance with the Declaration of Helsinki [20]. Ninety-five Japanese ladies participated with this study. The inclusion criteria were as follows: age groups between 40 and 60; having at least one menopausal sign within the Menopausal symptom level (MSS) (score >1). The exclusion.